LOCUS       CO961322                 472 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20e07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q18231 Q18231 SIMILAR TO 40S RIBOSOMAL PROTEIN
            S30/UBIQUITIN-LIKE PROTEIN. [1], mRNA sequence.
ACCESSION   CO961322
VERSION     CO961322.1  GI:51334506
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 529
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 taaagccaaa atccaagaca aagaaggcat tccacctgac caacaacgat tgattttcgc
       61 cggtaagcaa ctcgaggatg gacgaactct agccgattac aacatccaaa aggaatcgac
      121 cttacacttg gttttgcgcc ttcgcggagg aaaggtccat ggttcgcttg ctcgtgccgg
      181 aaaggttcgc gctcagacac caaaggtcga aaagcaagaa aagaagaaga aaaagcgcgg
      241 acgtgcattc cgtcgtattc aatacaaccg tcgctacgtc aatgttgcaa tggctggacc
      301 aggccgcaaa cgcggaccca actcgaatgc tgcgtaactt ggaaaagatt aaatggattc
      361 attcgcatga attgatgttt ttgcaggaat cctttttatt ttctgttatt tgaaattcga
      421 taaaccaatt tgttgcaaaa aaaaaaaaaa aaaaaaaaaa ataaaaaaaa aa
//