LOCUS       CO961319                 511 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20e01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to WP:CE22635 Y57E12AL.A, mRNA sequence.
ACCESSION   CO961319
VERSION     CO961319.1  GI:51334503
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 523
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgatttgaaa gttccaaacg actccgattc cttcgtcgac caaaactgac accaaaaagg
       61 ttcatttagc tggcgatgag tctgtgcaac gcgaggcgcc tcaaattctt gcgccaggtg
      121 tgggacaggc caaccacgaa ctcctgatgc cggaaacccg tcgagttccc acaatgggcg
      181 attttcttac gcaacgccga attcttcacg ggaatgcgca agctgaccag tggggacacg
      241 aactcttcat tgccacccag ctgaaccgtg ggccgcctgg agcgttcgcc tcgaacaatc
      301 tgcacatgga gatgctgctg ggactggacg accgcatcaa ccttgaacat tttcgcgact
      361 gaaagagttt tgcggtttta attgcaaatt atgactgttt ttcgtttttt attgtaaggt
      421 tattgataaa gttacccatt tacccaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      481 aaaaaaaaaa aaaaaaaaaa aaaaaaccaa a
//