LOCUS       CO961317                 355 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20d11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O18236 O18236 Y57G11C.12 PROTEIN. [1], mRNA
            sequence.
ACCESSION   CO961317
VERSION     CO961317.1  GI:51334501
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 497
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 agatatccca cttccggtgt ttcgagaagt gctcaaaaag caattctcga agaattcgca
       61 attgtcagac ttgagagtaa tcgaccgtaa actagaagaa ggtcgtcaac acatggtctc
      121 ggtgaaatac ttctattata atgaatatca tgtgaggaac atgctgtttg ccgaaaacgt
      181 agagccgaaa ccgaaagact tcctttccaa gtttctgttg ggcaaagagt gacgagagca
      241 gacagcagag agattcttta ggatacctaa tgcaaaaatt gacttttttg atgcaaagtc
      301 agctctgtat acaacacaca tttgaaaaaa aaaaaaaaaa aaaaaaaaaa acaaa
//