LOCUS       CO961311                 548 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20d05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q93576 Q93576 F25H2.5 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961311
VERSION     CO961311.1  GI:51334495
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 654
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 agcagcgtgg ttataaactg gtggctatca aaatggtcaa agcaacgccc caacacttgg
       61 aagtgcatta tgaggatctg aaggacaaac ctttcttcgc tggattagtg aagtacatgg
      121 cttcagggcc agttgtggca atggtttggg aaggactcga tgttgtgaaa caaggtcgag
      181 ctatgctcgg agccacaaat ccactggtga gtgcagcagg aacaattcgt ggaagctact
      241 gtatggacat gggacgaaac atctgccacg gatcagacag tgttccatcg gcagagcgtg
      301 aaattgcaca ctggttcaag caggaagaat tggttgactg ggagccagca cagatcaact
      361 ggatctacga gtgaggaaca atctggaaga cgcccatctg cgataaggaa gaaagtcccg
      421 gataccaatt cacaagacca tttgatttcg aggatttctt attcttattg tgtcgaaact
      481 taaagttgac aatgttaaaa tttgtgttgt gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      541 aaaacaaa
//