LOCUS       CO961304                 621 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20c06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RS21_CAEEL P49197 PROBABLE 40S RIBOSOMAL PROTEIN
            S21. [1], mRNA sequence.
ACCESSION   CO961304
VERSION     CO961304.1  GI:51334488
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 acgacgcagg cgaaacttgt ggagctgtat gttcctcgca aatgctcttc atcttcgcga
       61 atcatcgccg ctaaagacca cgcctcgatc cagctggaca ttgttggcgt aaacccggaa
      121 accggtcgta tggacccgag caaaacgacc aaatatgcga tctgtggaaa gctccgtttc
      181 atgggcgagt ccgacgattg catcctgcgt ttggcacaga aagatggaat tgttccagaa
      241 aagatgtaat cgtctgcgcg atccaaaatt gcgatttgtt gactgtttct gataaatgtt
      301 gtcgctataa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      361 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaagg aaaaaaaaaa aaaaaaaaaa
      421 acccccggcc cggaaactct tcccccccca ggggggaatt tgttaaaatt cccccaactg
      481 gggggcccct taaaatgtct ttaggggggc ccatttcccc caaaaagaga cctaaaaaaa
      541 cttccggggc ccccttttta acccctccgg gggggaaaaa ccggggggta acccaaataa
      601 ccccccccgc cccccccccc c
//