LOCUS       CO961303                 805 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20c05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:DYHC_CAEEL Q19020 DYNEIN HEAVY CHAIN, CYTOSOLIC,
            mRNA sequence.
ACCESSION   CO961303
VERSION     CO961303.1  GI:51334487
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 861
            Seq primer: SL1 primer
            High quality sequence stop: 801.
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ctacttcgcc gcctcatgca atttacaaga ggcactcaga cgtccgatgc tctgctcatg
       61 ttggcttaaa aagcactatt ctccggtcgc gcaaaaggag ttggaagatt acgtcggcca
      121 aagattgcga cagttctacg aagaggagtt ggatgtcaaa ctcgtcatgt acgaccaaat
      181 gctcgatcat gtgctccgca ttgaccgtgt cttccgtcaa cctcaaggcc atctactcct
      241 gattggcgtt tcgggctcag gaaagaccac cctatcgcgt tttgtcgctt ggctgaacgg
      301 atttttgaca gtccagctgg aagtcaaact tgcacgtcgc gttgaggagg caattattgt
      361 gtggatcagg attctcaagc aaagtcgcga tgagttggag gacgagcgcg aagcaaacat
      421 caacccggaa atattgccaa tttccctgga gattcgaatt caaagagact cttcttcaac
      481 attcttctct tccttacaac tggaaggcgc ccactctggt cgagcgctcc gtgctggtcg
      541 acagacgcgc ctcccgatct gggagcacct ctgcaagtcg ggcactcgcc aatcccatat
      601 tcgttcaaac gatgtgggcc gcgtctaaaa tcctgtccgg acggaatgct tccggcgaga
      661 gtgtctacct caaaatggac tcgaaaaagg aattggtgac aagagaagaa gagaattttt
      721 ggttcttgtg cacgaagcgc tgcttatgtg atttccaagt ctcattttcc atcccaattt
      781 ccataataaa aatgtaactt tnaaa
//