LOCUS       CO961300                 452 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20b12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:CYC1_ASCSU P92504 CYTOCHROME C, TYPE-1. [1], mRNA
            sequence.
ACCESSION   CO961300
VERSION     CO961300.1  GI:51334484
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 539
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gactccatgg ccacaaagac aggaccaacc ctgaacggga tttggggaag gaaaagtgga
       61 caggtacccg gattcgacta ttctgccgcc aacaaaaata agggtgttgt ttggaatcgc
      121 gaaacgctct tcgaatacct gctcaatcca aagaaatata tcccggggac gaagatggtt
      181 tttgcagggc tgaagaaggt cgacgaaagg gcggatctca tcaagtacat cgaggttgaa
      241 agtcagaaac cgccagcatg atgaattgac gcatgagagg ttcatcgccc gtccacgaca
      301 aacaggatag tttatagtca gtcacgtgcg agacacttgg ttaaaattgt ttttcaccta
      361 gacattttca cactctgagg ccctcgtttc taagaaattg ttagttcgaa aaaataaaat
      421 tcgttgacaa aaaaaaaaaa aaaaaaaaca aa
//