LOCUS       CO961296                 581 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20b08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961296
VERSION     CO961296.1  GI:51334480
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 789
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgttggcaga aaaaattgac gtccttattt caaaaatcga aatcgataaa aatggccaac
       61 aacgagaagc gattcgcggc gctcgaacaa gctgaccaac aacttcggcg agagctgcag
      121 gagcttcgcc aactggtcat gcagcgtgga ggaacagagc gcaaccagca agtcgaggag
      181 cttcgtctac gagctgagca ggccgaacag cgagctgagc aagccgagga agaactgcaa
      241 cggatgcagc aacagcgtca acgagagcgt caacgagagc gtcggcaaaa gctgcaaaag
      301 atgcagcaaa ttcgtcaacg agctgagcaa gccgaagcac tggttcgcca gctccaggga
      361 ccgacaacct caagcagagg attgcttcag gcaaacaact ggactgggat gctgcagaat
      421 gtggttgaac atccgccatc aatgactggg ccggggcacg aaaacgtggt tgaacatccg
      481 ggaccgtcca cctcaagcgg aggagtgctt ccggcagact ggactgagat gctgcagtac
      541 atggttgaac atccggcaca atcggccgag ccggagcgca c
//