LOCUS       CO961294                 608 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20b06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL2B_CAEEL P48162 PROBABLE 60S RIBOSOMAL PROTEIN
            L23A 1. [1], mRNA sequence.
ACCESSION   CO961294
VERSION     CO961294.1  GI:51334478
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 628
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggcagcagca gcgaaaagcc cgcagcggcg catccgagtg cggccgcgaa gaaaacggcg
       61 aagccaaaag acaaaaaggc tgtcgcgaag gcggtcgacg cgaagaagaa ggtggtgaag
      121 ggccaaaaca cgacgcacaa gaagaaggtg cgcacctctg tgcacttcta tcgcccgtcc
      181 acgctcaaaa cgccgcgagc gcccaagttc ccgcgcaagg cggtgcccaa gcgcaacaag
      241 ctggactcgt tcgcgatcgt caagaacccg ctgaccaccg agtcggccat gaagaagatc
      301 gaggacacca acacgctcgt gttcgtcgtg gacgtgaagg ccaacaagta ccagatcaag
      361 aacgccgtca aaaagctgta caacatcgac gtgcaaaagg tgaacacgct gatcacgccg
      421 aaccacacga agaaagccta cgtccggctg gcggccgact tcgacgcgct ggatgtggcg
      481 aacaaaatcg gaatcatctg agcaattccg cgcacccgac ggacacaaag ttttgttgtt
      541 ttgctccgag gagaagtata aaatttgtta tttgcaaaaa aaaaaaaaaa aacaaaaact
      601 cgagacta
//