LOCUS       CO961293                 531 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20b03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YV31_CAEEL Q20516 HYPOTHETICAL 6.8 KD PROTEIN
            F47B7.1 IN CHROMOSOME X. [1], mRNA sequence.
ACCESSION   CO961293
VERSION     CO961293.1  GI:51334477
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 537
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgcttctgtt cgcctccgtg ctcttgggca ccctcctttg ttccgcctac ggcgactcag
       61 ttgccgttga gaaggacggc tccatcgctt ctctcggcgt cggagacgag ggcaccgcgt
      121 tggagaagtt cctgacccgc acgaagcgaa gcagcggcag atggaccacc cagcagatca
      181 tcgagtgcat cttctgcata ttcatcccgc ccgtcgccgt cctgatccac gggggccacg
      241 agtgggtgct gcacctgatc atcaacatcg tgctctggat cttgggctgg attccgggca
      301 ccatccacgc cttctggtac tgcttcttcc gttgagccca atccccctct cttcttcaat
      361 actcctctac ccccggttat aagcattttg cgttcccttc ctgcttctag tagtgtgact
      421 ttttggctgt tgcccaccac caaatccatc cactcatgaa tttctcaacg accgataaat
      481 ttataaataa actgattcca tttaaaaaaa aaaaaaaaaa aaaaaaacaa a
//