LOCUS       CO961290                 635 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20a12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL15_CAEEL P91374 60S RIBOSOMAL PROTEIN L15. [1],
            mRNA sequence.
ACCESSION   CO961290
VERSION     CO961290.1  GI:51334474
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 728
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 atcatgggcg catacaaata catgcaagag atctggcgca agaagcagtc ggacgtgatg
       61 cgctacttgc tgcgcattcg ctgctggcaa taccgccaaa tggctgccat tcaccggtgc
      121 agccggccga cccgtccgga gaaagcgcgt cgtctcggct acaaggccaa gcaaggttac
      181 gtcatctatc gtgtgcgcat ccgccgcggt ggccgcaagc gtcaagtgac caagggtcag
      241 acctacggaa agccgaagac acacggagtg aaccagttga agaacacgcg cagtcttcag
      301 gccgtcgctg aagcacgaat tggacgtcgt tgtggagagc tgcgtgtgct caactcttac
      361 tgggtgggcc aggactccac gtacaagttc tacgaagcga tcctggtcga tccgttccac
      421 aaggtcattc ggcgcaatcc ggacacgcaa tggatcacca aaccggtgca aaagcaccgc
      481 gaacagcgtg gactcacatc ggccggacgc aagagccgtg gcctcggcaa gggcatcaag
      541 tacaccaaga ccaagggcgg ctcgcgccac aagtgctgga agcgacagaa caccatcgag
      601 ttccaccgaa agcgctaaat gggaaaatgc cgatt
//