LOCUS       CO961289                 625 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20a11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:GSHD_CAEEL O62327 PROBABLE GLUTATHIONE PEROXIDASE
            R05H10.5, mRNA sequence.
ACCESSION   CO961289
VERSION     CO961289.1  GI:51334473
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 769
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcgacaagta ccgcggcaag gagctgatgg tcgtgaaagt ggcgtcccag tgcgggctga
       61 ccgagagcaa ctacaaccag ctgaaggaga ctctggacaa atacaaggag aagggactcg
      121 ccatagccgc attcccgtgc aaccaattca atggacagga gcccggctgc cctatcgaca
      181 tcaaggaaac tgtgaacgtg aaatacaact ttgagccgga cttgtacgac aaagtcgacg
      241 tcaatgggga cagcgcacac cctctgtgga aatatctgca gaaggagaag ggaggcatcc
      301 ttggctggga caaaataaaa tggaacttca ccaagttctt gatcgaccgc aacggaaacg
      361 tggttgaccg ttatgccccg accacagaac cgaaaaagat tcacggcgac attgagaagc
      421 tgttggccta gatgaacgat cattatttga tcgcactttt aatccgaatt tgtgattccg
      481 gtttccctaa gtcctaacgc gactccagcg agcataccta ttacatgtgt agaaacgaat
      541 tggacccctc catccgtcac caaacaacaa tcgtactctt tgacaccgta gtaaactccg
      601 ggtgatcaac gaatgattct ccaca
//