LOCUS       CO961286                 454 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20a08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL23_CAEEL P48158 PROBABLE 60S RIBOSOMAL PROTEIN
            L23. [1], mRNA sequence.
ACCESSION   CO961286
VERSION     CO961286.1  GI:51334470
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 562
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgccgataat accggagcaa agaatctgtt tgtcattgcc gtgtacggga ttcgcggtcg
       61 gcttaaccga cttccaagtg ccgccgtcgg agacatgttt gtgtgttccg tcaaaaaagg
      121 caaaccagag ctgcgcaaaa aagttctcca agccgtagtt gtccgccagc gaaaacaata
      181 tcgtcggaag gatggcactt tcatttactt cgaggacaac gccggagtta tcgtcaacaa
      241 caaaggcgag atgaagggct cggcgattac cggcccagta gccaaggagt gtgccgacct
      301 ttggccgaag atcgcctcta atgcgagttc tattgcttga acggttgcgc atattgttga
      361 aacaaagcaa ctttgttaaa ctggttatat aaaaaaaaaa aaaaacaaaa aaaaaaaaaa
      421 aacaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//