LOCUS       CO961285                 634 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20a07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q18878 Q18878 C56A3.2 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961285
VERSION     CO961285.1  GI:51334469
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 646
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttgttctcct tttgtgcttc tgttcccttg gctcagcgat caggactcag tctgctgctg
       61 taaaaggtcg cttgatgtgt ggtgcggcac cggcgagaaa tgttcgagtc aaactgttcg
      121 atgaggattc tggacccgac cctgacgacc aattagacgc aggctacacg gactcaaatg
      181 ggaacttcca gctgaagggc tccaccaccg aagcaaccaa catcgacgtc gtgttcaaga
      241 tctaccacga ctgcgacgat gggattaagc ctggctctcg caaaatcaag ttcaagattc
      301 ccaactcgta cgtcagtgag ggagccaccc ccaaaaaaac atttgacatc ggcacgctga
      361 atctggagac catcttcgcc aacgaggaga gggagttgat cgtgagcaag aaaaggcgac
      421 acttgcacca ctaccggggc ggcgagaaat tcaacttttt cgttcgtctc taggtggcaa
      481 agaaagaaga atttggataa tggataagcc aaaacaaaca aaacaataca aatcgaccct
      541 ctttcgactt gtcaaattgc tttttaattg atttgcaata aagtttttgc accaaaaaaa
      601 aaaaaaaaaa aaaaaaaaaa aaaaaaaaac caaa
//