LOCUS       CO961284                 641 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20a06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to contains LTR7.t1 MSR1 repetitive element, mRNA
            sequence.
ACCESSION   CO961284
VERSION     CO961284.1  GI:51334468
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 taatgtaatt ttgatggaat cgtaaagcga caccagctac atatttgtgt aaagtaaatt
       61 ctgcagccga tagatacttt gcgattttcc tgcgtctgtt caaattcaaa tattcaaata
      121 cggaaatata aattacggtt tgctttaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      181 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      241 aaaaaaccca acccccaaac atttttcccc ccaaggggaa aatttccaaa aatccccccc
      301 acggggggcc cccccaaaca accctaaaag ggccccattt cccccaaaag ggaccccttt
      361 aaaatttccc ggcccccctt tttaaacccc ctggcgggga aaaccccggg gtttccccat
      421 tttaaccccc ttgccccccc cccccctttc ccccccgggg aaaaaaaaaa aaaacccccc
      481 ccctttcccc ccccccaaat tttccccccc cgaaagggga aagggccccc cccttaaccc
      541 cccctttaac cccggggggg gggggggttc cccccccggg gaccccccac tttgcccccc
      601 ccctaccccc cccctttttt tttttttccc ctttttttcc c
//