LOCUS       CO961278                 555 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19h11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9VP65 Q9VP65 CG12975 PROTEIN, mRNA sequence.
ACCESSION   CO961278
VERSION     CO961278.1  GI:51334462
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 563
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gcaatgaagc tgttgatgcc aatttcttct catcccactt cctaaagggt gtgaataccg
       61 gttatccgct gatattgcgt gcaaccaaaa ttgagagagt ggacgccaac ttcaacgcag
      121 agttcgttaa gaagatgatg ccacgcctgg actaccaggc tcttttgcaa gctgccaagg
      181 tggtggacca gagcgcagtg gcggaactgc ccgaagagct aaacgtcgag tgggagaaag
      241 acgaggagct gctaaaaaag atacattcgc tgttgatgag ctacgagatt gtggagggcg
      301 agctggagtg ccccgaaaca catcacgtgt tcaacatccg agagggcatt cccaatttcc
      361 tggtcggcgc tgacgaggtc gcctaggatt cggaccttgg aatcaaggtc tgaaggcaaa
      421 ttccatgttt ctgttcccaa taatccatct ctgcaatttt ttgagtttat atttgagtcc
      481 gcattaaaca catattcaga ccaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      541 aaaaaaaaaa ccaaa
//