LOCUS       CO961277                 550 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19h10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9VP65 Q9VP65 CG12975 PROTEIN, mRNA sequence.
ACCESSION   CO961277
VERSION     CO961277.1  GI:51334461
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 566
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aagctgttga tgcacaattt cttctcatcc cacttcctaa agggtgtgaa taccggttat
       61 ccgctgatat tgcgtgcaac caaaattgag agagtggacg ccaacttcaa cgcagagttc
      121 gttaagaaga tgatgccacg cctggactac caggctcttt tgcaagctgc caaggtggtg
      181 gaccagagcg cagtggcgga actgcccgaa gagctaaacg tcgagtggga gaaagacgag
      241 gagctgctaa aaaagataca ttcgctgttg atgagctacg agattgtgga gggcgagctg
      301 gagtgccccg aaacacatca cgtgttcaac atccgagagg gcattcccaa tttcctggtc
      361 ggcgctgacg aggtcgccta ggattcggac cttggaatca aggtctgaag gcaaattcca
      421 tgtttctgtt cccaataatc catctctgca attttttgag tttatatttg agtccgcatt
      481 aaacacatat tcagaccaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      541 aaaaaccaaa
//