LOCUS       CO961276                 586 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19h09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961276
VERSION     CO961276.1  GI:51334460
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 587
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gagtctgctt tttcgcctcg acatggacaa cgtcagccta aaaagcagca gccaaagaag
       61 agaggcagca tgaaggagaa cagctagaga tggttggatc gcgggcaaac tgctacagca
      121 ctcttaattc gggccagact gaagctcgct cgtcaagtga tcaaagcatt gataacgagg
      181 aggagcagca agatgtggat tttgaggaga cattcaacgc cgtcaacata gagaggatcg
      241 agggactttc gcgtgaagag ataagccggg agctcctcaa cagcgagaag gacaatcgac
      301 atttgcagca acgtgtcatc agcctgaaag aaagtcacga gcagttggtc cttgagaata
      361 tgcagttaaa agctgtgctt gcagagaacg ggatcgaggt gaatacaacg actgatgaca
      421 agaaatgagg agacaaatga tgctatacca ccgatcaatt tcattttcct accattgcgg
      481 aatatacatc atcatttaca aatttagtgc caccttaggc gcattttcaa ataaataaat
      541 atgcaagttc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aacaaa
//