LOCUS       CO961274                 544 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19h06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RS23_CAEEL Q19877 PROBABLE 40S RIBOSOMAL PROTEIN
            S23. [1], mRNA sequence.
ACCESSION   CO961274
VERSION     CO961274.1  GI:51334458
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 595
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgcgtaagct gaagaatcac cgtcaggaac agcgctggaa tgacaagcgc tacaagaaaa
       61 ccaactcggg aactcgctgg aaggccgatc catttggagg agcttgcatg gccaagggca
      121 ttgtgctcga gaaaatcgga gttcaagcca agcaaccgaa ctcggctatc cgtaagtgta
      181 gtccgtgttc agttgatcaa gaacagcaag aagatcacag ccttcgttcc aaatgacgga
      241 cgtttgaact tcattgagga gaacgacgag gtccttgttt ccggcttcgg ccgaagtgga
      301 cacgccgtcg gagatattcc tggagtgcgt ttcaaggtgg tcaaagttgc caacacatct
      361 cttctggcgt tgttccgcgg aaagaaagag cgaccacgtt cataaagccg cacaagttgc
      421 agaaacagat cacggacgga tttgttatca ctgttgatgt ttaagtaaat tggaaaaatc
      481 aaaaagaaaa aaacaaaaaa agaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaac
      541 caaa
//