LOCUS       CO961271                 851 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19h03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to WP:CE20835 F49H12.5, mRNA sequence.
ACCESSION   CO961271
VERSION     CO961271.1  GI:51334455
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 814.
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tctcgtccta ctgttcgccg tttgtgcact cgcttcattg gtcaatggcc ggggaagagt
       61 cgaaggcaca gattgacgag aacgaggtgg tcgagccgag cgcggatgga ccggatgcgg
      121 ctcaggaccc ctcgcatggt tttggcgccg acattgactg ggtgccgtgg ccgcaggcgg
      181 tgtcggtagc actcgacctg aacagaccca tcttcctgct catccacaag agctggtgtg
      241 gcgcgtgcca attgctcaaa aagagcttca acacgtccac aatgcgcgcc gagctggttg
      301 agttgagcgc ccagtttgtg atggtgaaca ccgaagacga tgaggagccg caagaggaag
      361 agtacgcgcc cgacggaggc tacatcccgc gcctgttcat attgaacaag cagggaaaac
      421 gcctcgcagt ggacaacaag aagaactacc cgagcaatgc tgcgtatttc ccgcaagtgc
      481 cggacgtcgt caaggcgatg aagaagggac tggaactgtt ccagaaggag cagaagtccg
      541 acggagaggc tgctgaaggc gtgaaaaagg cgaagggaaa gacggagaag aaacaaaagg
      601 cgaaagaggg cgatgaaaag gcggctcaga aggccgcaaa gacgatggac aaacagccca
      661 aggcggagga ggagatggcg tcggacagcg acaaaaaagg tggatgtcca cacgcggcgg
      721 gcaaagcggc caagaaggcg aaggaagaga ggccaagaag aaggcggcag aggcgaacaa
      781 cgagaaggcc aagggcaaaa gtgaggcggc gaaaaatgag gcgaacgccc caaaagggcc
      841 aaaaagccga a
//