LOCUS       CO961270                 516 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19g12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RS25_CAEEL P52821 PROBABLE 40S RIBOSOMAL PROTEIN
            S25. [1], mRNA sequence.
ACCESSION   CO961270
VERSION     CO961270.1  GI:51334454
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 525
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttgattaaag tcaatgccac cgaaagcggc caaaaaggca gatcccaagg gcgggaaggc
       61 ggcccctgcg aagaagaagg agggaggagg aggcggaggg aaggccaaga agaagaagtg
      121 gtccaagggg aaggtgcgcg acaagctgac caacatggtg ctcttcgatc aggccaccta
      181 cgacaagctg tacaaggaga tcatctcggt gaagctgatc acgcccagtg ttgtgtccga
      241 acgactgaag gtgcgcgcct ctctggccaa ggctggcctc caggaactcc accagaaggg
      301 gctgatcaag tgcgtggtga aacacggctc ccagctggtc tacacgcgag ccaccaaagc
      361 ggaagagggc accgcctgag cgaattgatt taggacgaac attcaacaag gatttctcgt
      421 ccatctttgt gaactgttat tgttaaagtt ttttttacga tgaattattt ctattgttaa
      481 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa accaaa
//