LOCUS       CO961269                 549 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19g11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to contains element MER22 repetitive element, mRNA
            sequence.
ACCESSION   CO961269
VERSION     CO961269.1  GI:51334453
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 551
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 atccaaaatc cattcacgct gtcctttccg cgacaatcga ccactaaaat ggcccagttc
       61 ctgtacgcat ttctcgcctc cctactccta ggtgtggtgc tcttcgaccg aaatgcgatg
      121 ggacaacagt tcgcagagtt cggggttgcc ggccctgaaa tgatggacct ggactacggt
      181 agcatcaatg acattgagag ccttgtgaag agagcctcgt tggaccgatc ggcgatggcc
      241 agattcggca agcgcgcacc gttggaccgg tcggccatgg ccagattcgg caaacgcgcg
      301 ccactggacc gatccgcgat ggtcagattc ggaaagcgag cccctctcga ccgatcggca
      361 atggccagat tcggaaagtg aagtgacaga gcgagcggtg tggcgcattt tttataaata
      421 agtgtgaagg aagcaagcac gatctaacat gttcacacaa acacactttt ggatcaacag
      481 ttacgaataa acaacccttt ggaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      541 aaaaccaaa
//