LOCUS       CO961268                 661 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19g10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:IF52_CAEEL Q20751 PROBABLE INITIATION FACTOR 5A,
            mRNA sequence.
ACCESSION   CO961268
VERSION     CO961268.1  GI:51334452
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 648.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttttaattaa tcgataatgt cggacaccga acatgaccac gatttttcgg tagcagatgc
       61 aggcgccgct gccacttacc cgaaacaatg ttcggccttg cgcaaaaacg aattcgttat
      121 gatcaaggga cgtccgtgca aggttgtaga gatgagtact tcgaagaccg gtaaacacgg
      181 gcatgcaaag gtgcacctgg tggcgctcga tatcttcact aataagaagc ttgaagacat
      241 ttgcccttcc acccacaata tggaagtgcc ggttgtgaag cgcaaggagt accagcttct
      301 ttctgtcaat gaggacggtt ttgtcagcat gatggatttg gagagctgcg acaccaagga
      361 tgacttgcgt ttaccagagg gtgagattgg ggaccagata aaacaggcgt acgagaaaga
      421 cgagaacggc atacttgtca atgtggtgtc cgcttgcggc gaggaactat tctcgggtgg
      481 aatacatgcc aaatcgcgaa taattgttgc ccacggcagg cacgggagag acgaggatga
      541 gtttgggtga aaatggagga ggatggcgac gtaaaagaca gcattcgatc acaattctcc
      601 tctctctctc catttccttt tgttgtgtta tatcaatcga cgccaccgat ttcaattcat
      661 c
//