LOCUS       CO961267                 623 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19g09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q93576 Q93576 F25H2.5 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961267
VERSION     CO961267.1  GI:51334451
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 646
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tttatcgacc caaatacgct atgtctacta aaaaccatta acgcacattc atcgcgatca
       61 agcctgatgg tgtccaaggc ggtcttgtga gcaaaataat caaacaattt gagcagagtg
      121 gttataaact ggtggctatc aaaatggtca aagcaacgcc ccaacacttg gaagtgcatt
      181 atgaagatct gaaagacaaa cctattcttc cctggattac tgaaatacat ggcttcaggg
      241 ccaattgtgg caatggtttg ggaaggactc catgttgtga aactaggtcg atctatgctc
      301 ggatccacaa atccactggt gagtgcatca tgaacaattc gtggaatcta ctgtatggac
      361 atgggacgaa acatctgcca cggatcagac agtgttccat cggcagagcg tgaaattgca
      421 cactggttca agcaggaaga attggttgac tgggagccag cacagatcaa ctggatctac
      481 gagtgaggaa caatctggaa gacgcccatc tgcgataagg aagaaagtcc cggataccaa
      541 ttcacaagac catttgattt cgaggatttc ttattcttat tgtgtcgaaa cttaaagttg
      601 acaatgttaa aatttgtgtt gtg
//