LOCUS       CO961265                 676 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19g07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL2B_CAEEL P48162 PROBABLE 60S RIBOSOMAL PROTEIN
            L23A 1. [1], mRNA sequence.
ACCESSION   CO961265
VERSION     CO961265.1  GI:51334449
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 688
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 atatttaatc caattactcc cggataaatg gcaccgaaac cagcggcagc agcagcgaag
       61 aagcccgcag cggcgcatcc gagtgcggcc gcgaagaaaa cggcgaagcc aaaagacaaa
      121 aaggctgtcg cgaaggcggt cgacgcgaag aagaaggtgg tgaagggcca gaacacgacg
      181 cacaagaaga aggtgcgcac ctctgtgcac ttctatcgcc cgtccacgct caaaacgccg
      241 cgagcgccca agttcccgcg caaggcggtg cccaagcgca acaagctgga ctcgttcgcg
      301 atcgtcaaga acccgctgac caccgagtcg gccatgaaga agatcgagga caccaacacg
      361 ctcgtgttcg tcgtggacgt gaaggccaac aagtaccaga tcaagaacgc cgtcaaaaag
      421 ctgtacaaca tcgacgtgca aaaggtgaac acgctgatca cgccgaacca cacgaagaaa
      481 gcctacgtcc ggctggcggc cgacttcgac gcgctggatg tggcgaacaa aatcggaatc
      541 atctgagcaa ttccgcgcac ccgacggaca caaagttttg ttgttttgct ccgaggagaa
      601 gtataaaatt tgttatttgc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      661 aaaaaaaaaa accaaa
//