LOCUS       CO961263                 522 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19g04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O62337 O62337 R06C1.4 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961263
VERSION     CO961263.1  GI:51334447
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 523
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggtctacggt ttacgcaaag tttaatcgct cttttacaat tcgagaaaat gtcttcatct
       61 ggctacagcg tttacatcgg aaacctggct tatcaatcca cggagaacga cgtcggaaat
      121 ttgttctccc aatgtggaac cgtcaccaat gttcgcattg tgtacgaccg cgagaccaac
      181 cgtccgaagg gatttggctt ctgcgagttc caggaccagc aaggcgctca gaatgctgta
      241 aaccagatga acggaactga gcttcacggt cgcgcactcc gtgtgaactt tgccaacaag
      301 tagaggcatg gttgctgaga agccatggaa tgaatgcaaa caagactcat gatcgggcct
      361 tagaataatt ccaaatttaa aaccgtattt ttggattcaa tctttaagcc ctgatcaaac
      421 gttgttgcac ctttaactta ttacgcattc tcatgagcta aaataaactg actttgttaa
      481 aaaaaaaaaa aaaaaaaaaa aaaaaacaaa aactcgagac ta
//