LOCUS       CO961262                 739 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19g03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:BTF3_CAEEL Q18885 TRANSCRIPTION FACTOR BTF3
            HOMOLOG. [1], mRNA sequence.
ACCESSION   CO961262
VERSION     CO961262.1  GI:51334446
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 822
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgggctctcc tattcgttcc aacctacatt gccgtttaaa atgaatgccg agaaagtcaa
       61 gaagcttcaa caaaatgccg agcatgttcg tacaggagga aagggtactg cacgtaggaa
      121 gaagaaggtg gtacacaaga ccaccgcaat ggacgataaa aaaattgcaa agcaacctaa
      181 agaaactctc tgtgaccaac attccgggta ttgaagaagt caacatgatc aaggacgatg
      241 gaaccgttat ccatttcaac aatccgaagg tgcaagcctc tgtgccagca aacacttttt
      301 caatcactgg gactgcggaa aacaaacaga tcaccgacat gctgcctggc atccttaacc
      361 agttgggcgc tgaatctttg actcacttga agcggctggc caacaacact acccaattca
      421 aagccactga agatgacgat gtgccagatc ttgtcggaga ttttgaggaa gcttcaaaga
      481 acgagtcaaa gaccaccggg actgctgcgg acaccaatgt taatgccgtc gaataaagta
      541 gccaacttgc tgttgacgat ggaacaaatt gatcagattt tcactcttcc atcgacgaga
      601 aagttcatct tgttgttatt tttcatttgt tttaatcgaa aacaactgac acaaaaatgt
      661 tttcttggct tatcaaatgt tctgagttca cattgccagg agagcgatac attttttttg
      721 ttatttcacg acccttatt
//