LOCUS       CO961261                 718 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19g02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961261
VERSION     CO961261.1  GI:51334445
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tttgtcgttg ttctggccct cgccctgttg gcggccgccg cccacagtgc gccgattgct
       61 gtggagcccc agcagcagga ccagtcctcc gcctccgacg acgggaccct gagcgcgcag
      121 gagatcaccg cgaagctgga cgagttggcg gaccaggtgc actccaccct gctccagtac
      181 gtggaccgct acgtggacgc ggacgactcg ctggccgccg aactgctcga ccagtgcacc
      241 aagcagtgcg accagctgcc ccagctggca cagcgcgccc tccagtcctt caaacagatg
      301 ccggacgagc acaagcagac gctgaaggcg acgctgcgcc gctctttcga cgagctgatg
      361 cagttcggcg tcaaattcat ggacgtgtgg gcgcccaagt tcgacaacgc gctgaccgac
      421 aaggtggtct ccattctggt caagcaggcg gcagacacca ctcgcaagga cctgttgaac
      481 cgcatcgacc agttggacgt ggacgccgca gcgaacgctc tcaacgacct gttggcggac
      541 aaggtgcccc agatgtgcca cacccactgc gacatggccc acaagcgcgt ccaggacgac
      601 ttcgagcgcc tccaatcgac ctcggacgag ggcaagaagg cgctgaaggg cttcccgcgt
      661 ccgttggtgg acgacatgtt ccagctgggc cgcaactacg tgcaccaggg cgtcaacg
//