LOCUS       CO961259                 524 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19f12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:P90799 P90799 ATP SYNTHASE PROTEIN 9, mRNA
            sequence.
ACCESSION   CO961259
VERSION     CO961259.1  GI:51334443
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 538
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgctgccagt gctacgtgtc gctgttatgc cgatggctcg ccagccgctc ggccggctca
       61 cctgccttcc gatgcagtcg gcgctgattc agtgccaaaa gctgagcacc agtgcagtgc
      121 gccaggacat tgacagtgcg gccaagtaca ttggagcagg tgccgccact gtgggagtgg
      181 ccggttccgg agcaggtatt gggaacgtgt tcggctcgct gatcatcggc ttcgcgcgca
      241 acccgtcgct caagcaggcg ctcttctcct acgccattct gggctttgcg ctgtctgaag
      301 ccatgggcct gttctgtctg accatgggct tcatgatctt gttcgccttc tgaacagcac
      361 aattggcact gcacaggcgt ggaaccgttc tccaccagac cattatagat gttttctttt
      421 tgtattcttt tgttctaaat gatagcaaca taggatgttg tctttgactg aataaaggtt
      481 caagttgccc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa caaa
//