LOCUS       CO961238                 524 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19d08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:P90860 P90860 F36A2.7 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961238
VERSION     CO961238.1  GI:51334422
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 713
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ccaatttcta aattgggctt caccactcca cccagcctct tcggggtgcc ttctccgtgt
       61 tttcgcgatt cgaaaatttg ccagtggctg ttttggaacg gccgccaaag gctaagatcc
      121 aactgaaacc accaccaggc gagaagggct acatgaacta tgaacgtgac ttttcacgcg
      181 aatcacgtta tacaaagcca cataagaaga ccgacacatt tataagattt gtaacattta
      241 acgctcgcca tcacatggag ctctggccga tcgtatttct ctgcggtgtc tcttgttgcc
      301 tagtcgtggg catagcctac ataagttttg aaaaagtgga agtctggctt gacagaagca
      361 ccaagacccc accatgggat tgggcgcgca ttcgtgacaa atactggaga atgcaaacag
      421 tatttttcga tccacttggc gggacccacc agcgtataga aatcatggag aagctacagg
      481 atgagatgtt ggaggcggcc aagcagcgtg gaaccgcgat gccg
//