LOCUS       CO961235                 602 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19d05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O01868 O01868 CODED FOR BY C. ELEGANS CDNA
            YK71B8.5. [1], mRNA sequence.
ACCESSION   CO961235
VERSION     CO961235.1  GI:51334419
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 604
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gctaatttta cgcgtaaaat agcgataatg aaaactgaga cctgtgttta ctctggatac
       61 aagatccacc ctggacatgg caagcgcata gtgcgggcag atggaaagat tcaaatctat
      121 ctgagcgcta aatgccaacg cggtgctgca ctcaagcgca acccgcgtga tgtgccatgg
      181 actgtgcttt accggcgcaa gcacaagaag ggtgtgcacg cggaagaagg ccaacagaag
      241 aagcgcatga agcgcactgt ccaaatagtc tctcgaccca tcgccgattt gactgtggaa
      301 gcgttgttgg cacagcgaaa ccagaagcct gagttccgca aacagcagcg tgatgcagcg
      361 atcaaagcgg ccaaggaatc tgtacgcgca aagaaagagg aaaccaagaa gaaggccaaa
      421 ctggacaaaa aggcggcacc ccaaaagatg aaggcggcta agcctgtgaa agccccaaag
      481 gcacaagttg ggggcaaacg ttgaaaagag tttgattgtt gagtgttgat ccaatttgcc
      541 gatcattgtt gttaagcaca aaaataaaac agtttaccat aaaaaaaaaa aaaaaaaaac
      601 aa
//