LOCUS       CO961234                 657 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19d04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q18878 Q18878 C56A3.2 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961234
VERSION     CO961234.1  GI:51334418
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tttgttctcc ttttgtgctt ctgttccctt ggctcagcga tcaggactca gtctgctgct
       61 gtaaaaggtc gcttgatgtg tggtgcggca ccggcgagaa atgttcgagt caaactgttc
      121 gatgaggatt ctggacccga ccctgacgac caattagacg caggctacac ggactcaaat
      181 gggaacttcc agctgaatgg ctccaccacc gaagcaacca acatcgacgt cgtgttcaag
      241 atctaccacg actgcgacga tgggattaag cctggctctc gcaaaatcaa gttcaagatt
      301 cccaactcgt acgtcagtga gggagccacc cccaaaaaaa catttgacat cggcacgctg
      361 aatctggaga ccatcttcgc caacgaggag agggagttga tcgtgagcaa gaaaaggcga
      421 cacttgcacc actaccgggg cggcgagaaa ttcaactttt ttcgttcgtc tctaggtggc
      481 aaagaaagaa gaatttggat aatggataag ccaaaacaaa caaaacaata caaatcgacc
      541 ctctttcgac ttgtcaaatt gctttttaat tgatttgcaa taaagttttt gcacaaaaaa
      601 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaatt
//