LOCUS       CO961230                 659 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19c11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9RBG6 Q9RBG6 LOW-SPECIFICITY D-THREONINE
            ALDOLASE, mRNA sequence.
ACCESSION   CO961230
VERSION     CO961230.1  GI:51334414
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 757
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aataactttt ggccgcccta ctggccacag cgctcgaatc aagccctttg ccctttgacg
       61 gcaggcagta cccgtccatc aacctgaggc tcgcgggccg gccatcggcg gacgaaggcg
      121 cagaatcgag gggcgaccaa caggcgacgc ggccgcagcg ctacacggag ctcggacgcg
      181 tcgttagtgg acgggcagag gctgtaccgg ctggagagcg gcggccagcc ggagggtcgg
      241 tcctgcctgg tgcgcgtggc gaccggcact cggctgaccc tgtcccggat gggcaccgcc
      301 agccctgaca gctacgtgcg agtgctggcc ggctcatcgc cgctgcacat gcccttggga
      361 acgcgacgaa ttcacaaggg ccaactggac ttgtacggcc cctggcacgt gccctgtgag
      421 tcggcctacc tggtgttcag cacaggggac tcttcgccgc gggagtgggt cgaattccgg
      481 ctggagaggg agacgcagga ggacgccagg acgacccggc cgagggcggg ggagtgttcc
      541 gcagagggag cggtcaatca cgcggaccag gaattcgaag gaccttcagg ggactgaccc
      601 aaaggggcca attggagaca cccacaaatg ttcacttgtc ctacgtttgg acaaaactg
//