LOCUS       CO961229                 710 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19c10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O42697 O42697 RIBOSOMAL PROTEIN L37. [1] ;contains
            MER22.b1 MSR1 repetitive element, mRNA sequence.
ACCESSION   CO961229
VERSION     CO961229.1  GI:51334413
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gccgccaaaa tgacgaaagg aacccaatcg tttggtatgc gtcacaacaa gacgcacact
       61 ctgtgccgtc gttgtggccg ctcctcctac cacatccaga agcacacttg cgcgtcctgc
      121 ggttacccag ccaagcggaa gcgcacttac caatggtcgg tgaagtcgat tcgccgtcgc
      181 accacgggca ctggacggat gcgccacctg aagctggtgc agcgccgctt ccgcaacgga
      241 ttccgcgagg gcactgtggc caaatcgcag aagaagggca agagcgccta gacgccggag
      301 atttgaacca gaacattgtt ttgatcgatt gttgcgaaat tgttaattga gaaaacattg
      361 aaagagactc ccaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      421 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ccaatccccc aaaaaggttt
      481 ttcccccggg gggaatttcg gaaaaatccc ccccccgggg gggcccccga aggggccttt
      541 aagggggcca atttccccca aaagggatcc gaataaaatt tcttgggcct tttttttaaa
      601 aatccggggg gggaaaaccc cggggtttcc ccaattaaac ccccttgcac ccccccccct
      661 tttccccacc gggagaaaaa caaaaaagcc cccccccatt ccccctcccc
//