LOCUS       CO961228                 739 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19c09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL7A_CHICK P32429 60S RIBOSOMAL PROTEIN L7A, mRNA
            sequence.
ACCESSION   CO961228
VERSION     CO961228.1  GI:51334412
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ctgccggccg ctatgaagaa gaccgaggcc tccaagaaag tgacaaagaa cacgcttttc
       61 gagaaaagaa ctcgcaactt cgcaataggc caggacatca ttccgaagcg ggacctgact
      121 cgctttgttc gatggcccaa gtacatcctt ctccagcgcc agctggccgt tctgatgaag
      181 cgtctcaaga tcccgccgac catcaaccag ttcaactttg cattggacaa gcccaacagt
      241 gcgcaactgc ttaagctgct caacaagtac cgaccggaga cgcgtcagga gaagaaagaa
      301 cgactgcgcc agcgtgccca ggaccgggtc gagggcaaac aagagaaggt gaccaagcgt
      361 ccgccggtgg tccacttcgg gatcaaggac gtggtcaagc aagtggagaa caagaaggcc
      421 agtctcgtgg cgattgcaca cgatgttgac ccgatcgaga tcgtcatctg tctgccggcc
      481 gtctgccgca agtttggcgt gccctatgtc attgtcaagg ggaaggctcg ccttggacaa
      541 gttgtgaacc gcaagacctg ttccgctgtg tgcctgtcgg acgtgaaccc ggaggacaag
      601 aacgcactga agaacttggc cgaggtggcc tccaccaact tcaacgagcg cggagacgag
      661 atccgcaagc actggggcgg cggcatcatg tccgcgcgat cgcaagcccg catggccaaa
      721 ttgagaagtc gcgcgttaa
//