LOCUS       CO961225                 455 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19c02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:VATF_CAEEL Q23680 PROBABLE VACUOLAR ATP SYNTHASE
            SUBUNIT F, mRNA sequence.
ACCESSION   CO961225
VERSION     CO961225.1  GI:51334409
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 513
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgctaggagg cattggacag ctgaacaaag cacgcaaacc aaatttcctg attgttgaca
       61 agaataccag tatttgcgga aatcgaggaa gcattccgga ctttttgcac gcgggacgac
      121 atcgccatca tactgattca accagcacat cgcggaaatg atccggtacc aagtagacca
      181 acacgtggcc agcactccgg cagtgctgga aattccctcg aaagagatgc cgtacgacgc
      241 gtcgaaggac agtatattga acagggctcg tggcctcatc aatcctgaag atctcaaata
      301 gagagtttca aaacgaactg atgaagccca tttcattccg gatttcgacg aactattttc
      361 acattcacaa ctatagagcg caaaaaatcg atttttgtcc ataatagtta tcagagaata
      421 aaatacagga tatgaaaaaa aaaaaaaaaa acaaa
//