LOCUS       CO961221                 758 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19b05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961221
VERSION     CO961221.1  GI:51334405
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 759
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgtgagtgat aagttaatcg gtatttgaaa tggaaacgcc cgattctggg acatcttcgg
       61 ccgaagacac ttttgagcgg actttccgaa agccaattca attgggtgca ttccttaacg
      121 gtgtcagagc taatccggct ccaaatggtc caacacgtcg aatcggaacg attgtcgttg
      181 aatgcaaatc gtcacgtcaa ccacgaggaa tggattgtgt gatattcgaa aatggagatg
      241 cgacacccaa tctggaactt gaagattaca tcgaaaacaa agtggaacta tacaatgtgc
      301 ttatgcgtcg ggaacgcgct gccggtcaaa gaggccgacc atctctttcc ccgtttcgtt
      361 tgggcgacaa agccaatgtc aatgagaagg gcggagaaaa atcaggagga ctttcctacc
      421 atctaacggc gagtttgact gaaacacgca atccaaatac tcccgaagaa catatcgtgc
      481 gctacaacgc tcattttgag tacattgctc cgcctgaccc aaccaagcgc caggggacta
      541 aagagcatag aagtgacatg aaagaagaac caaagggagg gggatttgca ggagaagctg
      601 gtgggtctgg atcgagccag gtatgaagcg ggacctgaga cattgaattg ttgaaaataa
      661 tttataaaaa tcgatattca tttttaaaat gtgagttggt ggaataaagg agcatatacc
      721 taaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaacaaa
//