LOCUS       CO961219                 695 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19b03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961219
VERSION     CO961219.1  GI:51334403
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 749
            Seq primer: SL1 primer
            High quality sequence stop: 677.
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggtgaaatgg ccgcgaacat gaacgacgcc gacgctggtg acgctcaggt gcacttggaa
       61 caggacggaa agcgagtttt cggcgaaccc gccattcatg cgatcatccc gagcgagttc
      121 aaaaacatgg cttcagttgg acgttatagt gtagctgacg aagacggcgt tgttgtgaat
      181 gtagctgtga aagttgacgg gaaaccgtac tgcttcggcc gaaaactgcc gatcatgatc
      241 gacttcgacg ggtttgacga cgtgtcggtc ccgacgcgct attgctccaa aatgccgaac
      301 cagcgacttc gctttgtcgt gctcctatgc aatggaggcg taattgacgc aaatgccgtg
      361 gcaaaggatg cccgcgatga gttcgaggaa tggcgcactg tgtttgggtg tcagatttct
      421 gagatgggaa atcagggatg cggaatgccg cttttgcaag tcaccattcc attcgagaac
      481 tttatcggcg agaaatttta cgaacttctg gaggcgatgg atgcgatcat caacgcaaac
      541 gatccgaaca aatctaacag ctctggtggt tctggcgtcg tcaatctgct ggcgccgatc
      601 gagcagtgat ttcctgcgaa aaaagcagtt ttgttgttaa cgtatattgt tggcaaaata
      661 taaatgtttt gttggtcnaa aaaaaaaaaa aaaaa
//