LOCUS       CO961211                 618 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe19a05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O01542 O01542 CODED FOR BY C. ELEGANS CDNA
            YK66C9.5. [1], mRNA sequence.
ACCESSION   CO961211
VERSION     CO961211.1  GI:51334395
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 627
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gtctcgcact actgccggag gtattggttt ccaggttgtg gccaagcagg cgagcaaata
       61 cagcaaccag gggggagaga tgtgcctggc ctggatcaag aaaatgagcg gcgagaatat
      121 cagcaccaac ggcgaccgtg agaacttcta caaattgcta aaagacggca cactactgtg
      181 caagctggcc aacgggattc aggcgggctc cgtcaagaag atccagcagc cgactggcaa
      241 cttcgcctgc atggagaaca tcaacgcttt cgtgtcgttc gcgaagagcc agggagtgcc
      301 caccgaggag accttccaga gcgtggactt gttcgaggca cgtgacctct actccgtgtg
      361 catgaccctg ttgtcgttgg gccgaattat ggagaagaag ggaaagccga acccattctc
      421 tggatgaaga gtagaagtga gtgcagcaaa agacggacag agcatgtgct atcgctccca
      481 ttcgagaact ccccgttttg cgaattttct ccccgtgtgc gaccttgcaa caataccgag
      541 gcgttaactg ttttcccctc tctctcctct caaactgtgg gcatttgaaa atagcgatgc
      601 cggaaaaaaa aaaacaaa
//