LOCUS       CO961204                 489 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18h04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O01542 O01542 CODED FOR BY C. ELEGANS CDNA
            YK66C9.5. [1], mRNA sequence.
ACCESSION   CO961204
VERSION     CO961204.1  GI:51334388
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 659
            Seq primer: SL1 primer
            High quality sequence stop: 464.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cggcgaccgt gagaacttct acaaattgct aaaagacggc acactactgt gcaagctggc
       61 caacgggatt caggcgggct ccgtcaagaa gatccagcag ccgactggca acttcgcctg
      121 catggagaac atcaacgctt tcgtgtcgtt cgcgaagagc cagggagtgc ccaccgagga
      181 gaccttccag agcgtggact tgttcgaggc acgtgacctc tactccgtgt gcatgaccct
      241 gttgtcgttg ggccgaatta tggagaagaa gggaaagccg aacccattct ctggatgaag
      301 agtagaagtg agtgcagcaa aagacggaca gagcatgtgc tatcgctccc attcgagaac
      361 tccccgtttt gcgaattttc tccccgtgtg cgaccttgca acaataccga ggcgttaact
      421 gttttcccct ctctctcctc tcaaaactgt ggcatttgaa aatagcgatg ccggaataaa
      481 tggccaatt
//