LOCUS       CO961194                 650 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18f12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9SNZ1 Q9SNZ1 UBIQUITIN PRECURSOR, mRNA sequence.
ACCESSION   CO961194
VERSION     CO961194.1  GI:51334378
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcctcaacgt tactcctcca aaaagctatc tacaaccaaa atgcaaatct tcgtaaagac
       61 tttgactggc aagaccatta ccctggaagt ggagggatcg gacacaatcg agaacgtcaa
      121 ggccaagatc caggacaagg agggcattcc gcccgaccaa cagcgtctga tcttcgccgg
      181 gaaacagctc gaagacggtc gcacgcttgc ggactacaac attcaaaagg agtccactct
      241 ccatttggtg ttgcgtctcc gcggaggaat gcagatcttt gtcaagactc tcactggaaa
      301 gaccattacc ctggaagtcg agggttctga caccatcgag aacgtcaagg ccaagatcca
      361 ggacaaggaa ggcattccgc cggaccagca gcgtctgatc ttcgccggaa agcagctcga
      421 ggacggtcgc acccttgcgg actacaacat ccagaaggaa tccaccctcc atctggtgtt
      481 gcgtcttcgc ggtggttccg cctgagtcac agaacgcgca gaggaatatg gcgtcttgag
      541 atgtggacca attttaattc ctgttctgat cacacgaaac acaaatccac atgttttacg
      601 atatacaata aacagcagaa aacaacctac attcattaag taatcctgct
//