LOCUS       CO961193                 650 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18f11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL1X_HUMAN Q02543 60S RIBOSOMAL PROTEIN L18A. [1],
            mRNA sequence.
ACCESSION   CO961193
VERSION     CO961193.1  GI:51334377
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 653
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgattcgcaa tgcctattaa agcatcagga gaaacgttga agaaatttat cgtagttggt
       61 cgcaaacttc cgacggagaa tgaccaaaat ccagcactgt ataagatggt tatctttgca
      121 agcaaccacg ttgttgcaaa gtcccgcttc tggtatttca ccagcatgtt gcgtcgtgtg
      181 aagaaaactc atggtgaaat catctcttgc actgagtcat tcgagaagaa gcctggctct
      241 gttaaaaact ttggcatctg gcttcgttat gactctcgaa ccggccacca caacatgtat
      301 cgcgagtacc gcgatgttac ggaggcaggt gcagtaactc agtgttatcg tgatatgggt
      361 gctcgtcatc gtgcacaagc agaccgcatc caaattatca aggtccagac aatcaaagtg
      421 gaggactgca agcgtgctgg tgtgaagcaa ttccacaact cgaagatccg gttcccgttg
      481 ccgcatcgcg tgaatgccaa acggcgcaag atggctccat tcaccactca gcgtccgaag
      541 acccactttg cttgaattgg caatcgttac acattgttgc tggaccgtat ttgtttcatt
      601 tcaatatggt tgaaagttat gaattgttaa aaaaaaaaaa aaaaaacaaa
//