LOCUS       CO961191                 575 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18f08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q21930 Q21930 R11D1.8 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961191
VERSION     CO961191.1  GI:51334375
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 591
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggagaagaga gatataatac agccatggtc gcgcatcaac aacgcatcta gctacattca
       61 gtggcaagtg atccgcaaga acagcgcgtt catcaagcgc caacgcggca ttccgaagca
      121 gttcagcaca gaaccgttca acatggcccg tgtgaacagc atccgccaca acggaatgat
      181 caaccggaag gcggtggacg tacagccggc caagggcggg aagggagtca ctgtgaccat
      241 caagaagaag aggaactcca cccagcccgc aaagtcgacg gtggtgacca cgctgagcaa
      301 ggacagccgt gcagtgatcg cctcgatcgg aaatgcgatc ggcgcgtaca agtcctctca
      361 ggcgaaactg gcccagcgtc gcgcgtcgca gatcctacgc tcacaaaaag tgaaggcaaa
      421 gccggtggca gcgaagaagg gcaaaactga gtaaaagaat gagagatgca aggggagaac
      481 cgactgaatg attccggaga ggaattttgt tgttgttgtt ggatgaaatg ttaagtaaaa
      541 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa acaaa
//