LOCUS       CO961189                 704 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18f06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9XW41 Q9XW41 W06D4.5 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961189
VERSION     CO961189.1  GI:51334373
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gcccctgaaa ggtgaaccaa tggccacaca gcgccttcag acaaagcgcc aaaccctgga
       61 tgaggcgtat gcgccgccgg caaacttcct ggagatcgaa gtggtcaacc cgatcacgca
      121 cggggtgggc aagaaccgtt acacggacta cgaagtcaga ctgcggacca acctgcccgt
      181 cttcaaacac aaggagtcca gtgtgcgtcg tcgctattcc gactttgagt ggctgaggaa
      241 tgagttggag cgcgacagca agatcattgt tcccccgttg ccgggaaagg cgttcaagag
      301 gcaactgccg ttccgcgctg acgacggcat tttcgaggag ggtttcatcg aggagcgacg
      361 caagggactg gagcaatttc tcaacaaagt tgccggccat ccgttggccc aaaacgagaa
      421 gtctctgcac atctttctgc aaatgaacga actggataag aattatattc ccggacgcat
      481 tcgaacagtc tgaagaacaa gggcaaacgg agaataatgt gatcgggccg atggaagaat
      541 ggtttttcgg acatcattta attttaacac acgtttgacc caaggtgatt tttctacata
      601 tagattgatt gattgattgc caagatcatc ttgcattgct ggaccttccc atctatgttt
      661 attggtttgc atatttgcac cttttgcgtc caaaaattga cccc
//