LOCUS       CO961181                 810 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18e07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9U5A0 Q9U5A0 THIOREDOXIN PEROXIDASE, mRNA
            sequence.
ACCESSION   CO961181
VERSION     CO961181.1  GI:51334365
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggcgacaatt cccggacttt gaggctgaga ccagtgatgg cccgatggcg agtttccacg
       61 actggatcgg cacggacagc tgggccattc ttttttcgca ccctgccgac ttcactccgg
      121 tgtgcaccac ggaattggct cgggctgccc agctggcgcc cgagttcgca cggcgacgag
      181 tgaaattggc ggcgttgagc tgcgattcgg cgggttccca tcgtaggtgg acggaagaca
      241 tcgccgcata cggtaatcga gttgctggcg atcaaagcac aaccatttcg ccagtgccga
      301 aatttccgtt cccgatcatt gccgacgaag accgccgttt ggccagccag ctgaacatgt
      361 tggacccgct caacgccgat ttggccggta agccgatgac agtgcgagca gtgttcattt
      421 tggatccgcc gaagcgactt cgcctctcca ttctctaccc agctagcacc ggacggaatt
      481 ttgacgagat tttgcgcgtg gtcgactcac tccaactgac cgacaagtgt atggttgcta
      541 cgccggaggg gtggaagcct ggttcttcgt gtgtggtgga accgagggtg gacgatgagc
      601 aggccaagca gctcttcggg gagggagcag tacagcattt ggatgttccc tccggaaaac
      661 ggtatttgcg gatgctgacg ccgaagacgg aggaggactg actgaagagg tggatcctgt
      721 tttccatgaa aatgttaaac cttttatacg ttcgcacaca agtttccgat aaaaaaaaaa
      781 aaaaaaaaga aaaaaaaaaa aaaaaaaaaa
//