LOCUS       CO961175                 635 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18d11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961175
VERSION     CO961175.1  GI:51334359
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 641
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tacttcagat tgtttgtgca gcatattatt tcgaaatttg ccactttcga gctttgcatc
       61 agccaacaga ttggctagta attaccaagc ggcagtggca gtgaccgcaa caccaaattt
      121 gacgaagagt agggaagcaa aaaagaaaga gaaggatttc gaaaaactgc agaaatttta
      181 tcaagaaaga cccccaaaga acagaaagaa gatcgtggtc gctgatactg agatagacac
      241 tcacgagcct ggcaaagatg gccagggttg gatgaattgt gtctacgaga aattgcatgc
      301 agaataccaa gaacatgaga ctaggcagcg ccaagagagg cgaaaattgc gagcggagct
      361 gaaagatttt tgcataggaa gaacactgag gttaccacca gagcaggttc cattagaaga
      421 gatgttacca gaggaaaacg gtgatgccat aaaccttgtt tatatggctc gcagcaacca
      481 tgcgcgaaga accaacaaag gaaacttgat gaatgttgat ccggcgatcg atcgatagct
      541 gccaattcat tgttcatttt tagatcttgc tgttggtttg tgaatgtcgt ttatagaaaa
      601 tagttcaatg ctcaaaaaaa aaaaaaaaaa caaaa
//