LOCUS       CO961173                 704 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18d09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:S61B_HUMAN P38391 PROTEIN TRANSPORT PROTEIN SEC61
            BETA SUBUNIT. ;contains element MSR1 repetitive element, mRNA
            sequence.
ACCESSION   CO961173
VERSION     CO961173.1  GI:51334357
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 atttattacg aattaccagt gaaattttac gtatagctac tatggctgac tcagcaaaag
       61 tacctggcgg cgggaaggta gttcctcgca cggctgctgg tggagcactc agacaacggc
      121 gtggtgccgg tacgaccaac aacagaactc gcactaccgg cggcggtgga ggcggtggcg
      181 gaatgaacag tggaggacta tggcgcttct acacggaaga ctcgactgga ctgaagattg
      241 gaccggtccc tgtgctcgtc atgtcacttg tgttcatcgc cagcgttttc attcttcaca
      301 tctggggaaa atacagtcgc ggtcactgaa gctagaacac cacaagaaat ggacaaatcc
      361 ccccacccga ctgtaaccac aatttgttta ttttcttaaa caagtgatcc aagtgatatc
      421 ctggcaatct aaagctcttc ctgacataaa ggttatttat tctgcaaaaa aaaaaaaaaa
      481 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      541 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa cccccaaaaa ttttttcccc caaggggaaa
      601 atttcaaaaa aaccccacaa cgggggcgcc ccccaaacag tcattaaggg gccccctttc
      661 ccccaaaagg ggatttaaat aaatttcccg ggcccttttt ttaa
//