LOCUS       CO961171                 565 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18d07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q19830 Q19830 COSMID F26G1. [1], mRNA sequence.
ACCESSION   CO961171
VERSION     CO961171.1  GI:51334355
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 574
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tagatctatc caaagagccc aatctctgtc taacagcgtc cgacggccca attgcctgac
       61 aaagtccccc gaggacatag cccccacgaa aaattgaaga tgtgctgcgc gaagctggac
      121 aaggcagacg ccgactgcaa gagtcgcttc tgtgactgga acgcgctgag ctcgaacaca
      181 gttctcatgt tccttgccac ctgcgcccct agaggcccaa ccgttggcca aatgtgggac
      241 tgtgcatcca gccgtgggga ccacacccat tgctgcaaca gaaaaggggt gcaaccgggc
      301 tgcatggcct actgcgagac taccaagggg gtgcccacag actacttccg gtacttgttc
      361 tgtctgaacg acttcaacaa aatccgggac tgtttcttct accacctcat tgaccagccg
      421 aacatcaacg gggacctttg aaattgagag tccaacacaa atttatttct caccacaaat
      481 aaatatttaa aagtggaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      541 aaaaaaaaaa aaaaaaaaaa ccaaa
//