LOCUS       CO961169                 521 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18d05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:R13A_CAEEL Q27389 PROBABLE 60S RIBOSOMAL PROTEIN
            L13A. [1], mRNA sequence.
ACCESSION   CO961169
VERSION     CO961169.1  GI:51334353
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 698
            Seq primer: SL1 primer
            High quality sequence stop: 514.
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgtgaagcaa tcaacatctc cgggaacttt caccgttcta agctcaagtt ccttgcattt
       61 ctgcgcaagc gttgcaatgt gaagccatcc cgtggtccat tccattaccg ggcaccgtcg
      121 cgtatcttct ggcgcacggt tcgtggcatg gtccctcaca aaactgctcg tgggatgaaa
      181 gcgctgaaga aactgaaatg ttttgacggc tgtccccagc cctacgacac gaagaagaaa
      241 ttcgttctac ccaactctct tcgccacatc gcactcaagc cgcgtcgcaa atgctgcact
      301 gttggacgtt tggcgcacga ggtcggttgg cagtaccaag gcgtcgtgga gaaactggag
      361 gccaagcgca aagtgcgtgg tgccgccttc catgagaaga agaaggccga gcagaagctg
      421 aagaagcagg cattggagaa atcaaaatcc aagactgccc cgttcgacaa gattttggcg
      481 cagtacggat acgcctaaat tggcttctta tttgttgatg a
//