LOCUS       CO961166                 561 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18c12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O73666 O73666 40S RIBOSOMAL PROTEIN S11, mRNA
            sequence.
ACCESSION   CO961166
VERSION     CO961166.1  GI:51334350
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 609
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gccgaacaga ctgaacgcgc ttaccaaaag cagcccacgg tgttcctgaa tgacaaattc
       61 cgcactcagg gcattggcaa gaagccggcc aacaaagacc gatactggaa gaacgtcggt
      121 ctcggcttca agacgccgcg cgaggccatc gagggcactt acattgacaa gaagtgcccg
      181 ttcactggca atgtgtccat tcgaggccgc attctcaccg gggtggtggt caagaacaaa
      241 atgcagcgga ccatcgtgat ccgccgagac tacctgcact ttgtgcccaa gtaccgccgt
      301 tacgagaagc gtcacaagaa catgtccgtc cattgttcgc cttgcttccg tgacgtctcc
      361 gtcggagaca ttgtcactgt tggagagtgc cgcccgctga gcaaaactgt ccgcttcaac
      421 gtgctcgagg tgatcaaggc gagcggatca aagaaggcat tcgacaaatt ctgaacgagt
      481 tgatgccaat gcgatatcaa atggatgttt gttaaacttt acattgttgt tatttaaata
      541 aaaaaaatga accaaaaaaa a
//